Optogenetic Control of Non-Apoptotic Cell Death

We introduce optogenetic tools (designated LiPOP) that enable photoswitchable necroptosis and pyroptosis in live cells with varying kinetics. The LiPOP allow us to reconstruct the key molecular steps involved these two non-apoptotic cell death pathways by harnessing power of light. further demonstrate use LiPOP, itself or coupled bioluminescence, achieve optochemical killing bacteria cancer vivo. LiPOPs set stage for triggering necroptotic pyroptotic both vitro vivo will likely find studying role nonapoptotic within tumor microenvironment.